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| Registros recuperados: 101 | |
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| Tello Salgado, Isaac. |
| Actualmente se estudia la capacidad funcional a nivel molecular de los hongos basidiomicetos como modificadores de la respuesta biológica, dada la efectividad de los productos examinados de activar al sistema inmunológico, sin embargo, el origen geográfico de estos recursos, principalmente son asiáticos y europeos. Ganoderma es reconocido por su eficiente actividad en el tratamiento de varias enfermedades, principalmente las anticancerígenas y antitumorales, crece de manera silvestre en el área central de México, una de las más importantes, debido a la convergencia de las regiones Neártica y Neotropical, lo cual da como resultado un amplia diversidad de recursos genéticos. En la presente investigación se aislaron 66 cepas del género Ganoderma producto de... |
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Palavras-chave: Caracterización; Cultivo; Filogenia; Ganoderma; Recurso Genético; Characterization; Cultivation; Phylogeny; Genetic resource; Doctorado; EDAR; Estrategias para el Desarrollo Agrícola Regional. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/372 |
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| Ayala Torres, Ana María. |
| Un total de 129 aislamientos bacterianos obtenidos de la micósfera de esclerocios de Claviceps gigantea se evaluaron in vitro para determinar su capacidad de inhibir el crecimiento de Fusarium graminearum, F. subglutinans y F. verticillioides patógenos a maíz. Ensayos cualitativos en confrontación dual, resultaron en la selección de 22 aislamientos bacterianos con el mayor potencial antagónico hacia una, dos o las tres especies de Fusarium. Los aislamientos se identificaron molecularmente mediante secuenciación parcial del gen 16s ADNr. Las cepas con mayor grado de antagonismo pertenecen a los géneros Bacillus y Pseudomonas. La caracterización en la producción de metabolitos potencialmente involucrados en la actividad antagónica, mostró que el 100% de las... |
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Palavras-chave: Bacillus; Pseudomonas; Biocontrol; Metabolitos secundarios; Caracterización; Secondary metabolites; Characterization; Fitopatología; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2299 |
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| Costa, Geraldo Márcio da; UFLA; Paiva, Luciano Vilela; UFLA; Piccoli, Roberta Hilsdorf; Universidade Federal de Lavras; Figueiredo, Demétrio Junqueira; Universidade Federal de Lavras; Pereira, Ulisses de Pádua; UFLA; Silva, Nivaldo da; EV-UFMG. |
| Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit... |
| Tipo: Pesquisa laboratorial |
Palavras-chave: 5.05.02.03-4 mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests Doenças Infecciosas dos Animais mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276 |
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| Nascimento,Naysa Flávia F do; Rêgo,Elizanilda R do; Nascimento,Mayana F; Bruckner,Cláudio H; Finger,Fernando L; Rêgo,Mailson M do. |
| ABSTRACT The cultivation of potted peppers as ornamental plants has increased considerably throughout the planet. In Brazil, this crop is more recent and still lacks cultivars. In order to increase the available variability and obtain new cultivars, the interspecific hybridization is very useful in add a desirable attribute that occurs in one species to another species, resulting in a new cultivar of agronomic interest. The objective of this study was to characterize parents and interspecific hybrids based on 27 quantitative traits and evaluate their genetic diversity by multivariate procedures. Parents of seven pepper genotypes were crossed, and, seven hybrids were generated. The experiment was conducted in a completely randomized design. Data were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum spp.; Hybridization; Relative importance; Breeding; Characterization. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000300315 |
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| Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005 |
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| Maia,Clara Yohana; Soares,Nazaré Suziane; Castro,Ana Cecília Ribeiro; Queirós,João Ravelly Alves de; Aragão,Fernando Antonio Souza de; Bordallo,Patricia do Nascimento. |
| ABSTRACT Anthurium affine is an ornamental Brazilian native plant very appreciated due to its long-lasting durability. To explore the genetic diversity available, 21 A. affine half-sibling progeny were evaluated using morphoagronomic and molecular descriptors. The morphoagronomic descriptors used were: plant height, plant diameter, growth habit, leaf number, leaf blade length, leaf blade width, petiole length, petiole diameter, leaf margin undulation, general appearance; soil cover rate and Random Amplified Polymorphic DNA (RAPD) markers the molecular one. All the descriptors within the progeny showed variability and general appearance, leaf margin undulation and soil cover rate are the ones that most contribute to the plants divergence. From 45 RAPD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clustering; Characterization; Genetic variation; Ornamental; RAPD. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400410 |
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| Vanzetti,Leonardo Sebastián; Pflüger,Laura Alicia; Rodríguez-Quijano,Marta; Carrillo,José Maria; Helguera,Marcelo. |
| Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, different sets of PCR markers were used to characterize the genetic variability of waxy loci from 103 Argentinean bread wheat cultivars. For the Wx-A1 locus, Wx-A1a and a novel molecular allele designed Wx-A1g were detected. Wx-B1 locus showed three alleles (Wx-B1a, Wx-B1b, Wx-B1e), and Wx-D1 locus showed only the Wx-D1a allele. Novel single-locus allele specific markers for Wx-A1b, Wx-B1b and Wx-D1b null alleles were... |
| Tipo: Journal article |
Palavras-chave: Characterization; Molecular markers; Triticum aestivum L.; Starch; Waxy genes; Wheat. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100004 |
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| Purquerio,Luis Felipe V; Lago,Antonio A do; Passos,Francisco Antonio. |
| The occurrence of hardseedness and the low percentage of seed germination are major challenges when growing okra. For this reason, five elite lines of okra developed at the Campinas Agronomic Institute (IAC), three with angular pods (lines 8.1, 20.1.2 and 47.1) and two with cylindrical pods (lines 13.1.2 and 20.1.R), were evaluated for seed quality and compared to two commercial seed samples (Horticeres Sementes and Sakata Seed Sudamerica) of cultivar Santa Cruz 47. Seeds were field produced in an experiment set in the Technological Park of the Horticulture Center of IAC, in Campinas, São Paulo State, Brazil, in a randomized block design, with three replications and 20-plant plots. Fruits were harvested 25, 35, 45, 55, 65 and 75 days after anthesis (DAA).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abelmoschus esculentus Moench; Advanced generation lines; Characterization; Seed quality. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362010000200017 |
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| Palma Cancino, Davira Yolanda. |
| El tecolote moteado mexicano es una especie amenazada, en Estados Unidos y México. En México esta especie ha sido poco estudiada. Para localizar, registrar y caracterizar el hábitat de los sitios de descanso del tecolote moteado mexicano, así como para determinar su distribución potencial en áreas adyacentes, se condujeron muestreos en Tlachichila, Zacatecas. Para ello, se establecieron estaciones de llamado nocturnos en la que se emplearon imitaciones de sus vocalizaciones y búsquedas durante el día. Se muestrearon 7, 639 ha, y se localizaron cinco individuos subadultos en tres sitios de descanso (dos parejas y un individuo solitario). La caracterización del hábitat se realizó en sitios de uso y sitios aleatorios, en parcelas circulares de 20 m de... |
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Palavras-chave: Strix occidentales lucida; Caracterización; Hábitat potencial; Maxent; Characterization; Potential habitat; Ganadería; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2369 |
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| González Cerón, Fernando. |
| El presente estudio tuvo como objetivo caracterizar la infestación por garrapatas en un hato experimental de ganado Criollo Lechero Tropical (CLT) ubicado en la región central del estado de Veracruz, México; la información generada permitiría iniciar el registro sistemático de la infestación causada por éstos ectoparásitos para considerarla como una posible característica a ser incluida en un programa de mejora genética a mediano plazo. A 28 vaquillas (655±35 días de edad) y 16 toretes (741±89 días de edad) CLT se les retiraron del cuerpo todas las garrapatas saturadas (≥4.5 mm) considerando 18 regiones corporales para identificar la especie a la que pertenecían, ubicar las regiones corporales donde se localizaban, determinar la carga parasitaria y estimar... |
| Tipo: Tesis |
Palavras-chave: Criollo lechero tropical; Garrapata; Caracterización; Maestría; Ganadería; Tropical milking criollo; Tick; Characterization. |
| Ano: 2007 |
URL: http://hdl.handle.net/10521/1623 |
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| Miranda, Ordilena Ferreira de; de Souza, Saulo Eduardo Xavier Franco; Milan, Rodrigo José; Bueno, Aline Borges; Almeida, Marcilio de. |
| Psychotria viridis Ruiz & Pav. (Rubiaceae) occurs naturally throughout the Amazon and it is traditionally used by indigenous communities, being incorporated into religious use in urban contexts over the last few decades. It is known and cultivated in many regions of South America for possessing valuable bioactive alkaloids. In this paper, we described P. viridis leaf morphology, anatomy and histochemistry from three populations cultivated in the southeastern Brazil, in order to identify possible adaptations to local environment and management. All plants presented terminal stipules and basic morpho-anatomical patterns of leaves, consistent with most species of the genus, as heterogeneous dorsiventral mesophyll, uniseriate epidermis, presents large... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Characterization; Alkaloid; Trichomes; Glandular duct; Domatia. characterization; Alkaloid; Trichomes; Secretory duct; Domatia.. |
| Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/50369 |
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| Ayaad,Tahany Hassan; Al-Akeel,Rasha Khalifah; Olayan,Ebtisam. |
| ABSTRACT The present investigation deals with the isolation and characterization of a lectin from Aedes aegypti (Ae aegypti) female mid gut extract that agglutinates various mammalian red blood cells (RBCs) such as human three groups A, B, and O (RH+), mouse, rat, guinea-pig, sheep and goat erythrocytes. The highest activity of both crude and isolated mid gut lectins were detected against sheep RBCs. Using (NH4)2 SO4 fractionation, ion-exchange and mannose-CNBr-Sepharose 6B affinity chromatography techniques, Ae. aegypti midgut lectin (Aelec) was purified to homogeneity.Isoelectric focusing (IEF) and reducing SDS/PAGE revealed that the isolated mid gut lectin had isoelectric point (PI) of 5.90, and subunits approximate molecular weights of 35.50 and 27.35... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lectin; Purification; Characterization; Hemagglutination; Aedes aegypti; Mosquitoes. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600905 |
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| Melgarejo,Luz Marina; Vega,Nohora; Pérez,Gerardo. |
| Two lectins were isolated from Canavalia ensiformis and Dioclea grandiflora seeds. Gel filtration produced a fraction corresponding to Con A or D. grandiflora lectin while erythroagglutination assays revealed a distinct fraction presenting a lectin that agglutinates human red blood cells (RBCs) but not rabbit RBCs. Hydrophobic interaction chromatography showed that the latter fraction yielded a protein that readily agglutinates human erythrocytes; the lectin was also purified by affinity chromatography on Lac-Sepharose showing similar properties to that of the Phenyl-Sepharose-purified lectin. Despite minor differences (carbohydrate content or A1%1cm), the two lectins showed similar molecular properties in that they consisted of two non-covalently linked... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diocleinae; Leguminoseae; Characterization; Lectins. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000300006 |
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| Registros recuperados: 101 | |
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